Preparation method of physiological saline solution

Normal saline is also called sterile saline. Normal saline is a 0.9 percent sodium chloride aqueous solution. People who have studied chemistry should know more about this aspect. Normal saline has many uses and is generally used widely in medical treatment. The methods of preparing normal saline are also different, and the preparation ratios of normal saline for different uses are also different. Let us learn about it below.

Formula 1 Physiological saline solution for various animals

The saline concentration required for mammals is 0.9%. Weigh 0.9 g of sodium chloride, dissolve it in a small amount of distilled water, and dilute to 100 ml.

The saline concentration required for birds is 0.75%. Weigh 0.75 g of sodium chloride, dissolve it and dilute it to 100 ml with distilled water.

The saline concentration required for amphibians is 0.65%. Weigh 0.65 g of sodium chloride, dissolve it and dilute it to 100 ml with distilled water.

Formula 2: Ringer's saline solution

Sodium chloride 6.5 g Sodium bicarbonate 0.2 g

Potassium chloride 0.14 g Sodium dihydrogen phosphate 0.01 g

Calcium chloride 0.12 g

First, dissolve sodium chloride, potassium chloride, sodium bicarbonate, and sodium dihydrogen phosphate in a small amount of distilled water respectively, mix them, and dilute them to 980 ml with distilled water. Then take calcium chloride and dissolve it in 20 ml of distilled water, and add the calcium chloride solution drop by drop into the above solution, stirring while adding to avoid the formation of insoluble calcium phosphate precipitate.

This solution is used for cold-blooded animals, especially amphibians.

Formula Locke's Saline Solution

Sodium chloride 9.0 g Sodium bicarbonate 0.1-0.3 g

Potassium chloride 0.42 g Calcium chloride 0.24 g

Dissolve sodium chloride, potassium chloride and sodium bicarbonate in a small amount of distilled water respectively, mix them and add distilled water to 980 ml. Dissolve calcium chloride in 20 ml of distilled water and add it dropwise to the above solution.

Through the above introduction, everyone should have some understanding of the method of preparing normal saline. The method of preparing normal saline is also very important. I hope everyone can learn more about this aspect. Knowing more can also greatly increase our knowledge and experience.