What is the insulin testing method?

Insulin is an essential hormone for the human body, and its effect on lowering blood sugar is very significant. Not only does the human body have this hormone, but there are also hypoglycemic drugs such as insulin. It is also very important to detect the insulin content in the drug. There are many ways to detect insulin. The insulin content can be detected through bioassay methods, or it can be detected through human cells.

Insulin testing methods

There are two methods for detecting insulin content: immunological method and biological assay method. Among them, the immunological detection method uses protein polypeptide drug antigens to determine the monoclonal or polyclonal antibodies at the cluster site to specifically recognize the drug being tested, and then measures the insulin content through radioactive counting, colorimetry, etc. The disadvantage of the immunological method is that it detects the immune activity of protein peptides rather than the biological activity, so the error may be relatively large.

Biological method

The principle of biological detection is to measure the insulin content in the human body through the specific response of the body's internal and external tissues or cells to the tested drug. The biological detection method has very strict requirements on the detection conditions and detection process, and the accuracy of the biological detection method is higher than that of the immunological detection method.

Insulin content

Generally speaking, insulin testing must be done in hospitals, and we cannot test our own insulin levels ourselves. The insulin testing process is relatively strict and has high professional requirements. Therefore, if we want to know the insulin content in the body, it is best to go to a regular hospital for testing. The hospital's test results are relatively accurate. Testing insulin is very necessary both in daily life and in drug measurement. Although we cannot measure insulin by ourselves, it is still very helpful for us to understand the method of measuring insulin.

Effects of Insulin

Insulin is a protein hormone secreted by pancreatic beta cells in the pancreas in response to endogenous or exogenous substances such as glucose, lactose, ribose, arginine, glucagon, etc. Insulin is the only hormone in the body that lowers blood sugar, while promoting the synthesis of glycogen, fat and protein. Insulin measurement is the most reliable method for diagnosing diabetes and distinguishing diabetes types, and is also an important indicator reflecting the storage and secretion function of insulin cells. Clinically, by measuring the secretion levels and curve characteristics of the patient's insulin and C-peptide at various time points during fasting and after meals, we can understand the degree of the patient's pancreatic islet function failure and assist in determining the clinical classification of diabetes. The detection methods of insulin in the body can be summarized into two categories: immunological detection methods and non-immunological detection methods. Immunoassay methods include radioimmunoassay, enzyme-linked immunosorbent assay and luminescent immunoassay; non-immune detection methods include isotope dilution method and high performance liquid chromatography.