Human leukocyte antigen b27

Many patients will find a leukocyte antigen b27 test on their medical records when they go to the hospital for examination. Some people test negative and some test positive, and negative is a normal value. Human leukocyte antigen B27 is a type of cell in biological immunology, which is mainly related to diseases related to rheumatic joints. Therefore, it has become an important indicator for diagnosing rheumatic bone diseases. The editor will explain human leukocyte antigen B27 in detail.

Human leukocyte antigen B27 (HLA-B27) is a marker antibody for rheumatoid arthritis (RA) with high specificity and diagnostic significance for early rheumatoid arthritis or rheumatoid arthritis with atypical clinical symptoms. HLA-B27 is closely related to ankylosing spondylitis (AS). 90% of AS patients are HLA-B27 positive, while the positive rate in normal people is only 4% to 7%. However, not all HLA-B27 positive people will suffer from AS. According to statistics, about 20% of HLA-B27 positive people develop AS or other seronegative spondyloarthropathy, such as Reiter's syndrome, reactive arthropathy, psoriatic arthropathy, etc.

Normal value

Generally negative.

Clinical significance

Abnormal results: One of the common characteristics of seronegative spondyloarthropathies is that they are associated with HLA-B27. Among them, ankylosing spondylitis has the strongest correlation with HLA-B27, with 90% of patients being HLA-B27 positive. The HLA-B27 positivity rate of Reiter's syndrome is 37%-80%, and the positivity rate of reflective arthritis is about 30%-77%. When psoriatic arthritis and enterogenic arthritis are accompanied by involvement of the axial joints, the HLA-B27 positivity rate can reach 30%, while the HLA-B27 positivity rate of rheumatoid joints is the same as that of the normal population, about 4%-8%, so it is of great significance in the differential diagnosis of these diseases. People who need to be examined: Patients with ankylosing spondylitis and rheumatoid arthritis can be examined as a basis for treatment.

Precautions

Inappropriate people: No special requirements. Taboos before examination: serum is drawn in the morning on an empty stomach, and eating is prohibited. Requirements for inspection: Mix thoroughly immediately after sampling to avoid bubbles, which can cause hemolysis. Keep at room temperature, constant temperature is best, do not refrigerate or freeze.

Inspection process

Follow the routine venous blood collection method and then perform the test: use the chemical fluorescence method, combined with sequence-specific technology, and design a pair of specific primers and a specific needle after sequence comparison. This not only improves the detection sensitivity and accuracy, but also makes the detection simple and fast. After the reaction is completed, the analysis and interpretation are directly completed without the need for electrophoresis detection.

Related diseases

Reiter's syndrome, ankylosing spondylitis, rheumatoid arthritis, ankylosing spondylitis scleritis in children

Related symptoms

Obsessive-compulsive spondylitis, rheumatoid arthritis.